Investigation of bovine leukemia virus-induced leukemogenesis

我们从199个人类细胞系、100个人类血液和27个乳腺癌组织样本中提取了DNA。采用灵敏PCR检测人BLV的存在。PCR结果显示，所有人细胞株均为BLV阴性。我们在6份血液和5份肿瘤组织样本中观察到BLV基因特异性条带。为了证实PCR结果，我们采用了两种不同的高通量测序方法，即病毒DNA-Capture-Seq和LM PCR文库增强NGS。两种高通量测序均未检测到任何与BLV测序对应的单读。BLV检测结果显示没有BLV特异性序列的证据。血液和癌组织样本的阳性结果可能是实验过程中交叉污染造成的。

We extracted DNA from 199 human cell lines and from 100 human blood and 27 breast cancer tissues sample. Samples were subjected to detect BLV presence in human by sensitive PCR. PCR result showed that all human cell lines were BLV negative. We observed BLV gene specific bands in 6 blood and 5 cancer tissue sample. To confirm the PCR result, we performed two different high throughput sequencing method, namely viral DNA-Capture-Seq and LM PCR library enhancement NGS. Both of the high throughput sequencing failed to detect any single read mapped to BLV sequencing. BLV detection results indicate that there is no evidence for BLV-specific sequences. The positive result in blood and cancer tissue samples might be caused by the cross contamination during the process of performing the experiment.