CROSS-AXIS SYNCHRONOUS FLOW-THROUGH COIL PLANET CENTRIFUGE

交叉轴同步通流盘管行星轮的改进模型 先前描述的离心机（Z 01 HL 01462-06 LBC）已经过测试， 蛋白质分离，在分析和制备规模，使用 由聚乙二醇1000（PEG）和 磷酸氢二钾各为12.5%（w/w）。 四种混合物 稳定的蛋白质，包括细胞色素c、肌红蛋白、卵清蛋白和 血红蛋白在两个柱中以260 至670理论塔板（TP）。 进一步的研究表明， 蛋白质分离的相对低的效率可能是由于 蛋白质分子的大小和/或异质性，而不是高 聚合物相体系的粘度。 现行制度 应用于E.大肠杆菌蛋白，具有良好的效果。

An improved model of the cross-axis synchronous flow-through coil planet centrifuge previously described (Z01 HL 01462-06 LBC) has been tested for protein separations, in both analytical and preparative scale, using a two-phase polymer system composed of polyethylene glycol 1000 (PEG) and dibasic potassium phosphate each at 12.5% (w/w). A mixture of four stable proteins including cytochrome c, myoglobin, ovalbumin and hemoglobin were resolved in both columns at efficiencies ranging from 260 to 670 theoretical plates (TP). Further studies suggested that this relatively low efficiency of the protein separation may be due to the size and/or heterogeneity of the protein molecules rather than high viscosity of the polymer phase system. The present system has been applied to fractionation of E. coli proteins with promising results.