PHYSICAL CHEMISTRY OF BIOLOGICAL MACROMOLECULES

这个项目的目的是研究一个广泛的物理性质 各种生物大分子，目标是将这些 大分子的结构和功能的性质。 的 重点是这些相互作用的热力学 大分子及其分子大小和形状。 分析 超浓缩和数学建模是主要的研究内容 使用的技术。 对HIV逆转录酶的研究一直致力于 研究的热力学均匀缔合的 P66亚基，以及P66与P51的异质性关联 亚单位 对chromagranin A的研究已经指向 研究pH和钙离子浓度对 分子聚集性质的热力学，并朝着 研究这些相同的因素对不同的肽结构域的分子。 对DNA聚合酶-b的研究已被导向调查 分子的构象特性通过荧光和 超集中的研究，并对调查的相互作用， 酶与核酸。 亮氨酸拉链肽CEB-63的研究 已经被导向确定热力学参数 表征其可逆聚集。

The purpose of this project is to study the physical properties of a wide variety of biological macromolecules, with the goal of correlating these properties to the structure and function of the macromolecules. The emphasis is on the thermodynamics of the interactions of these macromolecules and on their molecular size and shape. Analytical ultracentrifugation and mathematical modeling are the principal research techniques used. Studies on HIV reverse transcriptase have been directed toward investigating the thermodynamics of the homogeneous association of the P66 subunit, and the heterogeneous association of P66 with the P51 subunit. Studies on chromagranin A have been directed toward investigating the effects of pH and calcium ion concentration on the thermodynamics of the aggregative properties of the molecule, and toward studying these same factors for various peptide domains of the molecule. Studies on DNA polymerase-b have been directed toward investigating the conformational properties of the molecule by fluorescence and ultracentrifugal studies, and toward investigating the interaction of the enzyme with nucleic acids. Studies on CEB-63, a leucine-zipper peptide, have been directed toward determining the thermodynamic parameters characterizing its reversible aggregation.