INTRAOCULAR CLASS II ANTIGEN EXPRESSION IN ENDOTOXIN-INDUCED UVEITIS

内毒素是一种来源于革兰氏阴性杆菌细胞壁的多糖。 细菌。当它被注射到老鼠的脚垫或眼睛里时，它会 在眼睛内引发炎症反应。这其中的机制是 炎症仍不清楚。然而，由于几种类型的前路 人类的葡萄膜炎似乎与革兰氏阴性细菌接触有关， 这被认为是人类前葡萄膜炎的相对模型，例如 发生在莱特氏综合症中。在本研究中，第II类基因的表达 研究了接受大肠杆菌感染的大鼠眼睛内的抗原。 用免疫组织化学技术检测内毒素。我们观察到， 睫状体和虹膜上II类抗原的表达先于 炎症细胞涌入眼睛，而炎症细胞 进入眼睛的主要是中性粒细胞和一些单核细胞。不是 炎性渗出液中可见T细胞。炎症性的 细胞浸润性，可被消炎痛或秋水仙碱抑制； 然而，这并没有改变虹膜对第二类抗原的表达。 或日记正文表明这一表达不是简单的结果 炎性浸润性疾病，但可能与 内毒素诱导葡萄膜炎的表达机制。皮质类固醇 能够抑制细胞的炎性浸润性和 II类抗原的表达。第II类抗原的表达 眼内的非淋巴样细胞在抗原中可能是重要的 或者可以简单地发出细胞表型变化的信号，因为 内毒素与细胞膜的相互作用。调查结果是 与被动和主动的II类抗原表达的比较 眼内阿瑟斯反应。内毒素对眼部的影响 用荧光分光光度法研究炎症，以验证使用 动物研究是一个有用的模型。牛睫状体上皮细胞的培养 并且发现只有在伽马干扰素存在的情况下才能表达II类。 大鼠睫状体上皮可作为抗原提呈细胞发挥作用。

Endotoxin is a polysaccharide derived from the cell wall of gram negative bacteria. When injected into the footpad or the eye of a rat, it will induce an inflammatory reaction within the eye. The mechanism of this inflammation is still unclear. However, since several types of anterior uveitis in humans appear to be linked to gram negative bacteria exposure, this is considered a relative model for anterior uveitis in humans such as occurs with Reiter's syndrome. In this study, the expression of class II antigens was studied within the eyes of rats receiving Escherichia coli endotoxin by immunohistochemical techniques. We observed that the expression of class II antigens on the ciliary body and iris preceded the influx of inflammatory cells into the eye and that the inflammatory cells that entered the eye were primarily neutrophils with some monocytes. No T-cells were present in the inflammatory infiltrate. The inflammatory cellular infiltrate, could be inhibited by indomethacin or colchicine; however, this did not alter the expression of class II antigens by the iris or diary body indicating that this expression is not simply a consequence of the inflammatory infiltrate but may be intimately involved with the mechanism of the expression of endotoxin-induced uveitis. Corticosteroids were capable of suppressing both the cellular inflammatory infiltrate and the expression of class II antigens. The expression of class II antigens on nonlymphoid cells within the eye may be important in antigen presentation or may simply signal a phenotypic change on the cells due to the interaction of endotoxin with the cell membranes. The findings were compared with the expression of class II antigen in passive and active intraocular Arthus reaction. The effect of endotoxin on ocular inflammation was studied using fluorophotometry to validate the use of animal studies as a useful model. Bovine ciliary epithelium was cultured and found to express class II only in the presence of gamma interferon. Rat ciliary epithelium can function as an antigen presenting cell.