HORIZONTAL FLOW-THROUGH COIL PLANET CENTRIFUGE WITH MULTILAYER COIL SET

该设备围绕该线圈保持一组四个相同的多层线圈， 柱保持器。这些柱子串联在一起， 总容量约200 ml。由于偏心定向， 多层线圈相对于保持器的轴线径向地 来自保持器轴的作用离心力保持固定相， 在线圈的每个螺旋匝中， 而线圈的行星运动产生两者的有效混合 溶剂相该系统提供了两相流的普遍应用 溶剂体系，包括水-水聚合物相体系， 大分子和细胞颗粒的分配。的能力 该装置成功地用于分离细胞色素c和 溶菌酶在由12.5%聚乙二醇组成的聚合物相系统中 1000在IM磷酸钾水溶液中。

The apparatus holds a set of four identical multilayer coils around the column holder. These columns are interconnected in series to make up a total capacity of about 200ml. Because of the eccentric orientation of the multilayer coils with respect to the axis of the holder, the radially acting centrifugal force from the holder axis retains the stationary phase, either the lighter or the heavier phase, in each helical turn of the coil while the planetary motion of the coil produces efficient mixing of the two solvent phases. The system provides universal application of two-phase solvent systems including aqueous-aqueous polymer phase systems used for partition of macromolecules and cell particles. The capability of the apparatus was successfully demonstrated in separation of cytochrome c and lysozyme in a polymer phase system composed of 12.5% polyethylene glycol 1000 in IM potassium phosphate aqueous solution.