ANIMAL MODELS OF ATHEROSCLEROSIS

补充正在进行的研究，包括血管镜检查和激光组织 因此，有必要开发一种可繁殖的动物， 动脉粥样硬化模型。 为了测试原型多功能导管， 为了模拟单临床条件，我们采用了多种方法 动物实验室里的模型。 到目前为止，已经有14只小型猪 作为致动脉粥样硬化饮食（胆固醇水平500- 1，000 mg/dl）和血管内球囊内皮剥脱术 使用无菌手术技术在荧光透视下进行。 从单个 股动脉切开，颈动脉球囊剥脱， 双侧髂股动脉 术后立即获得的血管造影片 气球引起的气压伤显示受影响的血管痉挛 船舶. 我们已经检查了几只动物在一至三个月后， 在成熟过程中因各种原因死亡的初始程序 研究的阶段。 严重和晚期局灶性动脉粥样硬化病变 在球囊剥脱部位观察到钙化， 机化血栓和“人”样花药形态。 在 此外，我们还在主动脉和髂股动脉进行了球囊剥脱术， 20只新西兰白色家兔也接受致动脉粥样硬化饮食。 手术后数周死亡的几只兔子的尸检标本， 显示主动脉脂肪条纹病变较不严重， 髂血管的血流动力学显著异常。 我们认为 这两种动脉粥样硬化模型非常适合帮助研究 体内激光血管成形术的可行性。

To complement ongoing studies including angioscopy and Laser-tissue interactions, it has become necessary to develop a reproducible animal model of atherosclerosis. To test prototype multifunctional catheters in an attempt to simulate uniclinical conditions, we have employed a variety of models in the animal laboratory. Thusfar, 14 miniature swine have been placed as combination of atherogenic diet (cholesterol levels 500-1,000 mg/dl mg/dl) and intravascular balloon endothelial denudation performed under fluoroscopy utilizing sterile surgical techniques. From a single femoral arterial cutdown, balloon denudation was performed in carotid and iliofemoral arteries bilaterally. Angiograms obtained immediately after balloon-induced barotrauma revealed varked vasospasm of the affected vessels. We have examined several animals at one-three months after the initial procedure who have died for various reasons during the maturation phase of the study. Intense and advanced focal atherosclerotic lesions were observed at the sites of balloon denudation consisting of calcified, organized thrombus and "human" appearing antherosclerotic morphology. In addition, we have performed balloon denudation in the aorta and iliofemoral system of 20 New Zealand white rabbits also receiving an atherogenic diet. Necropsy specimens in several rabbits who died weeks after the procedure, revealed less intense fatty streak lesions in the aorta with more focal hemodynamically significant abnormalities in the iliac vessels. We believe that these two models of atherosclerosis are well suited to help study the feasibility of in vivo laser angioplasty.