A mollecular epidemiological study of genes enrolled in the development of athoroscrelosis or hypertension

对参与动脉粥样硬化或高血压发展的基因进行分子流行病学研究

• 批准号: 10470098
• 负责人: OMAE Kazuyuki
• 金额: $ 8.45万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10470098/
• 关键词: mollecular epidemiology; polymorphism; common disease; atheroscrerosis; hypertension

In this study, we performed genetic testing to the participants of 6 years follow-up study and investigated the significance of genetic factor on evolution of hypertension or atheroscrerosis. One hundred thirty five persons gave consent to genetic testing and 10 polymorphism of 10 genes including beta 3-adrenergic receptor (B3AR) Trp64Arg, uncoupling protein 1 (UCP1) A-3826G, beta 2-adrenergic receptor (B2AR) Gln27Glu, lipoprotein lipase (LPL) Hind III, apolipoprotein AI (ApoA1) Msp1, apolipoprotein B (ApoB) insertion/deletion, apolipoprotein C (ApoC) Sst1, choresteryl ester transfer protein (CETP) Taq1b, methylenetetrahydrofolate reductase (MTHFR) C667T, angiotensinoger (AGT) M235T were examined. Mean age of first study (S1) was 34.0 years old that of second study (S2) was 40.0 years old.A-3826G polymorphism of UCP1 gene showed borderline significance (P=0.056) in the BMI of S2. We could not see positive result for AGT on blood pressure or LPL, B3AR, UCP1 and B2AR on glucose metabolism. Msp1 polymorphism of ApoA1 gene was adopted in the multupe regression analysis of triglyceride (TG) at S1 and S2. In S1 Sst1 polymorphism of ApoCIII gene was also adopted. Taq1B plymorphism of CETP gene was adopted in LDL cholesterol examination of S1. Finally, we analyzed the data of beta which indicates atheroscrerosis of carotid artery. Taq1B polymorphig of CETP gene showed borderline significance (p=0.052) in S1 study.We also performed cross-sectional study composed of 70 males whose age was 20 to 25 years old. Trp64Arg polymorphism of B3AR gene may be one of the determinants of fat distribution, Hind III polymorphism of LPL gene showed positive relation with TG or chiromicron. Again, we could not see positive result for AGT on blood pressure,

本研究通过对6年随访研究的参与者进行基因检测，探讨遗传因素在高血压或动脉粥样硬化演变中的意义。135人同意进行基因检测和10种基因的10种多态性，包括β 3-肾上腺素能受体（B3 AR）Trp 64 Arg、解偶联蛋白1（UCP 1）A-3826 G、β 2-肾上腺素能受体（B2 AR）Gln 27 Glu、脂蛋白脂肪酶（LPL）Hind III、载脂蛋白AI（ApoA 1）Msp 1、载脂蛋白B（ApoB）插入/缺失、载脂蛋白C（ApoC）Sst 1、检测胆固醇酯转运蛋白（CETP）Taq 1b、亚甲基四氢叶酸还原酶（MTHFR）C667 T、血管紧张素（AGT）M235 T。第一次研究的平均年龄为34.0岁，第二次研究的平均年龄为40.0岁，UCP 1基因A-3826 G多态性对S2的BMI有边缘性意义（P=0.056）。AGT对血压及LPL、B3 AR、UCP 1、B2 AR对糖代谢均未见阳性结果。采用ApoA 1基因Msp 1多态性对S1、S2时甘油三酯（TG）进行多元回归分析。在S1中也采用了ApoCIII基因的Sst 1多态性。采用CETP基因Taq 1B多态性检测S1的LDL胆固醇。最后，我们对颈动脉粥样硬化的指标β进行了分析。在S1研究中，CETP基因Taq 1B多态性显示临界显著性（p=0.052）。B3 AR基因Trp 64 Arg多态性可能是影响体脂分布的因素之一，LPL基因Hind Ⅲ多态性与TG、染色体数呈正相关。同样，我们没有看到AGT对血压的阳性结果，