A mollecular epidemiological study of genes enrolled in the development of athoroscrelosis or hypertension

在这项研究中，我们对6年随访的参与者进行了基因测试，并探讨了遗传因素在高血压或动脉粥样硬化演变中的意义。135人同意进行遗传检测，检测了10个基因的10个多态性，包括β3肾上腺素能受体(B3AR)Trp64Arg、解偶联蛋白1(UCP1)A-3826G、β2肾上腺素能受体(B2AR)Gln27Glu、脂蛋白脂肪酶(LPL)Hind III、载脂蛋白AI(ApoA1)Msp1、载脂蛋白B(ApoB)插入/缺失、载脂蛋白C(ApoC)Sst1、舞蹈酯转移蛋白Taq1b、亚甲基四氢叶酸还原酶(MTHFR)C667T、血管紧张素转换酶(AGT)M235T。第1次研究的平均年龄(S1)为34.0岁，第2次研究(S2)的平均年龄为40.0岁。UCP1基因A-3826G多态性与S2的体重指数呈临界性差异(P=0.056)。AGT对血压无阳性反应，LPL、B3AR、UCP1、B2AR无阳性结果。采用载脂蛋白A1基因Msp1多态性对甘油三酯(TG)进行多因素回归分析。在S1中还采用了APOCIII基因的Sst1多态性。S1的低密度脂蛋白检测采用CETP基因Taq1B多态。最后，我们对反映颈动脉粥样硬化的β数据进行了分析。在S1研究中，CETP基因Taq1B多态性具有边缘意义(p=0.052)。我们还对70名20~25岁男性进行了横断面研究。B3AR基因Trp64Arg多态可能是脂肪分布的决定因素之一，LPL基因Hind III多态与甘油三酯、红细胞微球呈正相关。再一次，我们看不到AGT对血压的积极结果，

In this study, we performed genetic testing to the participants of 6 years follow-up study and investigated the significance of genetic factor on evolution of hypertension or atheroscrerosis. One hundred thirty five persons gave consent to genetic testing and 10 polymorphism of 10 genes including beta 3-adrenergic receptor (B3AR) Trp64Arg, uncoupling protein 1 (UCP1) A-3826G, beta 2-adrenergic receptor (B2AR) Gln27Glu, lipoprotein lipase (LPL) Hind III, apolipoprotein AI (ApoA1) Msp1, apolipoprotein B (ApoB) insertion/deletion, apolipoprotein C (ApoC) Sst1, choresteryl ester transfer protein (CETP) Taq1b, methylenetetrahydrofolate reductase (MTHFR) C667T, angiotensinoger (AGT) M235T were examined. Mean age of first study (S1) was 34.0 years old that of second study (S2) was 40.0 years old.A-3826G polymorphism of UCP1 gene showed borderline significance (P=0.056) in the BMI of S2. We could not see positive result for AGT on blood pressure or LPL, B3AR, UCP1 and B2AR on glucose metabolism. Msp1 polymorphism of ApoA1 gene was adopted in the multupe regression analysis of triglyceride (TG) at S1 and S2. In S1 Sst1 polymorphism of ApoCIII gene was also adopted. Taq1B plymorphism of CETP gene was adopted in LDL cholesterol examination of S1. Finally, we analyzed the data of beta which indicates atheroscrerosis of carotid artery. Taq1B polymorphig of CETP gene showed borderline significance (p=0.052) in S1 study.We also performed cross-sectional study composed of 70 males whose age was 20 to 25 years old. Trp64Arg polymorphism of B3AR gene may be one of the determinants of fat distribution, Hind III polymorphism of LPL gene showed positive relation with TG or chiromicron. Again, we could not see positive result for AGT on blood pressure,