A mollecular epidemiological study of genes enrolled in the development of athoroscrelosis or hypertension

在本研究中，我们对6年随访研究的参与者进行基因检测，探讨遗传因素在高血压或动脉粥样硬化演变中的意义。125人同意对β 3-肾上腺素能受体（B3AR） Trp64Arg、解偶联蛋白1 (UCP1) A-3826G、β 2-肾上腺素能受体（B2AR） Gln27Glu、脂蛋白脂酶（LPL） Hind III、载脂蛋白AI (ApoA1) Msp1、载脂蛋白B （ApoB）插入/缺失、载脂蛋白C (ApoC) Sst1、choresteryl酯转移蛋白（CETP） Taq1b、亚甲基四氢叶酸还原酶（MTHFR） C667T、检测血管紧张剂（AGT） M235T。第一项研究（S1）的平均年龄为34.0岁，第二项研究（S2）的平均年龄为40.0岁。UCP1基因A-3826G多态性在S2的BMI中具有临界意义（P=0.056）。我们没有看到AGT对血压或LPL， B3AR， UCP1和B2AR对葡萄糖代谢的阳性结果。采用ApoA1基因Msp1多态性对S1和S2的甘油三酯（TG）进行多元回归分析。S1中还采用ApoCIII基因Sst1多态性。采用CETP基因Taq1B多态性检测S1的低密度脂蛋白胆固醇。最后，我们分析了β数据，这表明颈动脉粥样硬化。在S1研究中，CETP基因Taq1B多态性具有临界性（p=0.052）。我们还对70名年龄在20 - 25岁的男性进行了横断面研究。B3AR基因的Trp64Arg多态性可能是脂肪分布的决定因素之一，LPL基因的Hind III多态性与TG或染色质呈正相关。同样，我们没有看到AGT对血压的阳性结果，

In this study, we performed genetic testing to the participants of 6 years follow-up study and investigated the significance of genetic factor on evolution of hypertension or atheroscrerosis. One hundred thirty five persons gave consent to genetic testing and 10 polymorphism of 10 genes including beta 3-adrenergic receptor (B3AR) Trp64Arg, uncoupling protein 1 (UCP1) A-3826G, beta 2-adrenergic receptor (B2AR) Gln27Glu, lipoprotein lipase (LPL) Hind III, apolipoprotein AI (ApoA1) Msp1, apolipoprotein B (ApoB) insertion/deletion, apolipoprotein C (ApoC) Sst1, choresteryl ester transfer protein (CETP) Taq1b, methylenetetrahydrofolate reductase (MTHFR) C667T, angiotensinoger (AGT) M235T were examined. Mean age of first study (S1) was 34.0 years old that of second study (S2) was 40.0 years old.A-3826G polymorphism of UCP1 gene showed borderline significance (P=0.056) in the BMI of S2. We could not see positive result for AGT on blood pressure or LPL, B3AR, UCP1 and B2AR on glucose metabolism. Msp1 polymorphism of ApoA1 gene was adopted in the multupe regression analysis of triglyceride (TG) at S1 and S2. In S1 Sst1 polymorphism of ApoCIII gene was also adopted. Taq1B plymorphism of CETP gene was adopted in LDL cholesterol examination of S1. Finally, we analyzed the data of beta which indicates atheroscrerosis of carotid artery. Taq1B polymorphig of CETP gene showed borderline significance (p=0.052) in S1 study.We also performed cross-sectional study composed of 70 males whose age was 20 to 25 years old. Trp64Arg polymorphism of B3AR gene may be one of the determinants of fat distribution, Hind III polymorphism of LPL gene showed positive relation with TG or chiromicron. Again, we could not see positive result for AGT on blood pressure,