１神経細胞における機能的ジーンサイレンシングの可視化

1 神经元功能基因沉默的可视化

• 批准号: 15F15711
• 负责人: 影山 龍一郎
• 金额: $ 1.47万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2015
• 资助国家: 日本
• 起止时间: 2015-07-29 至 2017-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15F15711/
• 关键词: Neurobiology; RNA dynamics; synaptic plasticity; learning and memory

The difficulties with defining a successful imaging protocol for the cell line used in this project have eventually been overcome and, although this took a little longer than expected, we have been able to proceed quite rapidly with the imaging experiments. The fellow has a manuscript in progress that will be able to completed after her return to Australia. As there is only have 1 month remaining on this fellowship, the fellow is reaching the end of experimental work here in Japan. The current status of her research is as follows.1) Submission of manuscript for a project begun in Australia and completed in Japan with new technique established in the host laboratory during year 1 of the fellowship. 2) Refinement of a protocol for confocal imaging of the human neuronal cell line being used for the current project. 3) Learned new Reprogramming techniques for analysing m6A RNA modification by next-generation sequencing data. 4) Learned protein analysis techniques that have revealed unexpected and exciting information about the translation control pathways used in response to neuronal activity. 5) Co-author on a manuscript about to be submitted by the host lab. 6) Manuscript in progress from own project.

为该项目中使用的细胞系定义成功的成像方案的困难最终被克服了，尽管这比预期的时间长一点，但我们已经能够相当迅速地进行成像实验。该研究员有一个正在进行的手稿，将能够完成后，她返回澳大利亚。由于这个研究金只剩下一个月，这个研究员在日本的实验工作即将结束。她目前的研究状况如下：1）提交了一个项目的手稿，该项目开始于澳大利亚，在日本完成，在研究金的第一年，在东道国实验室建立了新的技术。2)对用于当前项目的人类神经元细胞系的共聚焦成像的协议进行改进。3)学习新的重编程技术，通过下一代测序数据分析m6A RNA修饰。4)了解蛋白质分析技术，揭示了关于响应神经元活动的翻译控制途径的意想不到和令人兴奋的信息。5)合作撰写一份由主办实验室提交的手稿。6)正在从自己的项目中进行制造。

项目成果

Activity-dependent dynamics of mRNA expression and m6A methylation in human neuronal cells

人类神经元细胞中 mRNA 表达和 m6A 甲基化的活动依赖性动态

• 发表时间: 2016
• 作者: Belinda Goldie;Youqi Liao;Takayuki Ohara;Dan Ohtan Wang
• 通讯作者: Dan Ohtan Wang

University of New Castle(Australia)

纽卡斯尔大学（澳大利亚）

Conservative miRNA target analysis: are we limiting our discoveries of human neuronal function?

保守的 miRNA 靶点分析：我们是否限制了人类神经元功能的发现？

• 发表时间: 2015
• 作者: Belinda J Goldie;Dan Ohtan Wang;Murray J Cairns
• 通讯作者: Murray J Cairns