１神経細胞における機能的ジーンサイレンシングの可視化

为这个项目中使用的细胞系定义一个成功的成像方案的困难最终被克服了，尽管这比预期的时间要长一些，但我们已经能够相当迅速地进行成像实验。她正在写一份手稿，将在她回到澳大利亚后完成。由于该奖学金只剩下1个月的时间，该研究员在日本的实验工作即将结束。她的研究现状如下。1)在奖学金的第一年内，在澳大利亚开始，在日本完成，在东道国实验室建立新技术的项目稿件。2)改进当前项目中使用的人类神经元细胞系共聚焦成像协议。3)学习新的Reprogramming技术，利用下一代测序数据分析m6A RNA修饰。4)学习蛋白质分析技术，揭示了在神经元活动响应中使用的翻译控制途径的意想不到的和令人兴奋的信息。5)共同撰写一篇即将由主办实验室提交的论文。6)自己项目中正在进行的手稿。

The difficulties with defining a successful imaging protocol for the cell line used in this project have eventually been overcome and, although this took a little longer than expected, we have been able to proceed quite rapidly with the imaging experiments. The fellow has a manuscript in progress that will be able to completed after her return to Australia. As there is only have 1 month remaining on this fellowship, the fellow is reaching the end of experimental work here in Japan. The current status of her research is as follows.1) Submission of manuscript for a project begun in Australia and completed in Japan with new technique established in the host laboratory during year 1 of the fellowship. 2) Refinement of a protocol for confocal imaging of the human neuronal cell line being used for the current project. 3) Learned new Reprogramming techniques for analysing m6A RNA modification by next-generation sequencing data. 4) Learned protein analysis techniques that have revealed unexpected and exciting information about the translation control pathways used in response to neuronal activity. 5) Co-author on a manuscript about to be submitted by the host lab. 6) Manuscript in progress from own project.