VIRAL DETERMINANTS OF HIV-1 PATHOGENESIS BY CONSTRUCTION OF RECOMBINANT VIRUSES

虽然HIV-1和HIV-2在核苷酸水平上有40%-60%的同源性，但 病毒感染的发病机制有相当大的分歧。再一次，HTLV-II 和HIV-1都是嗜T细胞病毒，它们在 它们的目标细胞，前者导致宿主转化，并 后者具有明显的细胞病变效应。特异性病毒决定簇 可能控制这些病毒在宿主中的不同致病性 细胞。我们已经开始了涉及重组基因构建的研究 具有给定病毒类型的单基因替换的病毒。目标 目前正在研究的序列位于LtR、TAT、REV、 Env和整合酶基因。这些重组体病毒将被 进入宿主细胞以及这些替换对病毒的影响 将对复制进行研究。

Although HIV-1 and HIV-2 share 40-60% homology at the nucleotide level, the pathogenesis of the viral infections are fairly divergent. Again, HTLV-II and HIV-1 are both T cell tropic viruses that replicate very differently in their target cells, the former causing the host to become transformed and the latter having a marked cytopathic effect. Specific viral determinants may govern the different pathogenicities of these viruses in their host cells. We have initiated studies involving the construction of recombinant viruses that have a single gene replacement of a given virus type. Target sequences that are currently being investigated are in the LTR, tat, rev, env, and integrase genes. These recombinants viruses will be transfected into the host cell and the effects of these substitutions on viral replication will be studied.