LYMPHOCYTE MIGRATION IN EXPERIMENTAL AUTOIMMUNE UVEITIS

免疫诱导大鼠实验性自身免疫性葡萄膜炎（EAU）

Experimental autoimmune uveitis (EAU) is induced by immunization of rats and other experimental animals withS-antigens (a soluble antigen from the retina) is being investigated in this laboratory as a model of human intra-ocular inflammation. This experimental inflammation can be transferred from donor rats to naive recipients using lymphocytes harvested from the spleen or lymph nodes. Following harvesting of the cells from the donors and three days in culture with stimulating antigen, the cells are injected into the intra-peritoneal cavity and five to seven days later the recipient rats develop EAU. The disease can also be transferred using a T-helper cell line by intra-peritoneal or intra-ocular injection. The mechanism of transfer of disease in unclear. This work has used radioactively labeled lymphocytes to determine the fate of these lymphocytes after injection into the peritoneal cavity or blood during the process of the development of uveitis. The goal of this project is to understand the initiating mechanisms of inflammation in the hope that these mechanisms can be extended and applied to human inflammations. Thus far we have determined that only a small percentage of the lymph node lymphocytes injected into the peritoneal cavity actually reached the eye during the induction of EAU. Of 100 million cells transferred into the peritoneal cavity, approximately 5,000 reach the eye. Many more reach the spleen, liver and thymus however. The work is now being extended to study the migration patterns of intra-peritoneal or intra-ocular T-cells lines.