ANALYSIS OF TRANSCRIPTS INVOLVED IN GLYCOCONJUGATE SYNTHESIS IN D MELANOGASTER

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 这项合作涉及果蝇聚糖相关基因的转录分析。 我们之前已经对从几种哺乳动物样品的细胞或组织中分离的RNA进行了qRT-PCR分析，C. elegans和斑马鱼，但这个与果蝇的项目将平台扩展得更远。 Tiemeyer博士和Katoh博士提供了一份D.用于引物设计的黑腹果蝇基因和用于引物设计和验证的果蝇基因组DNA。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This collaboration involves transcript analysis of glycan-related genes in Drosophilia melanogaster. We have previously preformed qRT-PCR analysis on RNA isolated from cells or tissues from several mammalian samples, C. elegans and zebrafish, but this project with Drosophilia extends the platform even further. Dr. Tiemeyer and Dr. Katoh provided an initial list of D. melanogaster genes for primer design and fly genomic DNA for primer design and validation.