IMMUNOPATHOLOGY IN THE EYES WITH EXPERIMENTAL AUTOIMMUNE UVEITIS

免疫活性细胞的识别和地形图定位 啮齿类动物眼部驻留细胞表面标志的变化 对实验性自身免疫性葡萄膜视网膜炎(EAU)进行了分析 免疫组织化学研究。在EAU的早期阶段，T辅助/诱导者 是眼睛中的主要细胞；在晚期，一个近亲 T抑制细胞/细胞毒细胞明显增加。T淋巴细胞 特异性是针对结合在细胞表面的小段抗原。 主要组织相容性复合体(MHC)分子出现在 特化的抗原提呈细胞表面。MHC-II类分子的表达 在RPE、视网膜等眼部驻留细胞上观察到抗原 内皮细胞、角质形成细胞、成纤维细胞和睫状上皮。这两个 外周血中浸润性细胞亚群与II类抗原表达 眼部驻留细胞可以被各种免疫调节剂改变， 其中一些-庆乃宁，FK506，15-脱氧精氨酸，白细胞介素2-PE40， 和S抗原对EAU的免疫病理效应进行评价。 EAU小鼠眼部的临床和免疫病理检查 表现为慢性复发性局灶性肉芽肿性炎症 视网膜和脉络膜中的血管炎。视网膜下发育 也可能发生新生血管。浸润性细胞主要是 巨噬细胞和T辅助细胞/诱导物(CD4)。MHC-II类分子的表达 抗原被立即限制在眼部驻留细胞中 炎症部位。由于EAU的慢性和复发性，小鼠 可能成为研究人类眼部炎症的更好模型。 实验性内毒素诱导性葡萄膜炎(EIU)是一种前葡萄膜炎模型 人类患葡萄膜炎。这种炎症的机制仍不清楚。 因为最近有研究表明磷脂酶A2的激活 可能在这种疾病的开始和传播中起到作用，我们 研究了一种新的合成肽和一种PLA2抑制剂的作用， 安非他明，对大鼠的EIU。

Identity and topographic localization of immunocompetent cells and alteration of surface markers on ocular resident cells in rodents with experimental autoimmune uveoretinitis (EAU) were analyzed by immunohistochemical studies. In the early stage of EAU, T helper/inducers were the predominant cells in the eye; in the late stage, a relative increase of T suppressor/cytotoxic cells was observed. T lymphocyte specificity is directed to small fragments of antigen bound to cell surface major histocompatibility complex (MHC) molecules which are presented on the surface of specialized antigen-presenting cells. Expression of MHC class II antigens was observed on ocular resident cells such as RPE, retinal endothelium, keratocytes, fibroblasts, and ciliary epithelium. Both the infiltrating cell subpopulation and the expression of class II antigens on ocular resident cells can be altered by various immunomodulating agents, some of which-Qingaining, FK506, 15-deoxyspergualine, interleukin 2-PE40, and S-antigen-were evaluated for their immunopathological effects on EAU. Clinical and immunopathologic examinations of the eyes of mice with EAU demonstrated a chronic relapsing focal granulomatous inflammation and vasculitis in the retina and choroid. Development of subretinal neovascularization may also occur. The infiltrating cells were mainly macrophages and T helpers/inducers (CD4). The expression of MHC class II antigens is confined to ocular resident cells immediately at the inflammatory sites. Due to the chronicity and recurrence of EAU, the mouse may serve as a better model for human ocular inflammation. Experimental endotoxin-induced uveitis (EIU) is a model for anterior uveitis in humans. The mechanism of this inflammation is still unclear. Because recently it has been suggested that activation of phospholipase A2 may play a role in the initiation and propagation of this disease, we studied the effect of a novel synthetic peptide and a PLA2 inhibitor, antiflammin, on EIU in rats.