TWO PHOTON ACTIVATION AND IMAGING OF CHR2 EXPRESSING EXCITABLE CELLS

表达 CHR2 的兴奋细胞的两次光子激活和成像

• 批准号: 7722584
• 负责人: TATIANA B KRASIEVA
• 金额: $ 0.64万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-15 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722584
• 关键词: Calcium; Cells; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Image; Institution; Lasers; Research; Research Personnel; Resources; Sapphire; Source; United States National Institutes of Health; research study; two-photon

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Here, we plan to investigate the possibility of two-photon activation of ChR2 expressing excitable cells by using tunable femto second Ti: Sapphire laser (860 to 960 nm). In order to visualise activation of ChR2, calcium imaging of activated cells will be carried out. These experiments will yield important parameters of ChR2 activation that are critical for two-photon activation.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在这里，我们计划调查的ChR 2表达可兴奋细胞的双光子激活的可能性，通过使用可调谐飞秒钛：蓝宝石激光（860至960 nm）。为了可视化ChR 2的活化，将进行活化细胞的钙成像。 这些实验将产生对双光子激活至关重要的ChR 2激活的重要参数。