IMMUNOPATHOLOGY IN THE EYES WITH EXPERIMENTAL AUTOIMMUNE UVEITIS

免疫活性细胞的鉴别和拓扑定位， 啮齿类动物眼常驻细胞表面标志物的改变 实验性自身免疫性葡萄膜视网膜炎（EAU）进行了分析， 免疫组织化学研究。在EAU的早期阶段，T辅助/诱导物 是眼内的主要细胞;在晚期， 观察到T抑制细胞/细胞毒性细胞增加。T淋巴细胞 特异性针对与细胞表面结合的抗原的小片段 主要组织相容性复合体（MHC）分子，其存在于 特殊抗原呈递细胞的表面。MHC II类分子的表达 在眼驻留细胞如RPE、视网膜色素上皮细胞、视网膜色素上皮细胞和视网膜色素上皮细胞上观察到抗原。 内皮、角膜细胞、成纤维细胞和睫状上皮。两者 浸润细胞亚群和II类抗原的表达 眼内驻留细胞可被各种免疫调节剂改变， 其中包括庆大宁、FK 506、15-脱氧精胍啉、白细胞介素2-PE 40、 和S抗原对EAU的免疫病理学作用。 EAU小鼠眼的临床及免疫病理学观察 表现出慢性复发性局灶性肉芽肿性炎症， 视网膜和脉络膜血管炎视网膜下发育 也可能发生新血管形成。浸润细胞主要为 巨噬细胞和T辅助/诱导物（CD 4）。MHC Ⅱ类分子的表达 抗原仅限于眼驻留细胞， 炎症部位。由于EAU的慢性和复发性， 可以作为人类眼部炎症的更好模型。 实验性内毒素诱导的葡萄膜炎（EIU）是前葡萄膜炎的模型。 人类的葡萄膜炎这种炎症的机制尚不清楚。 因为最近有人提出磷脂酶A2的激活 可能在这种疾病的发生和传播中起作用，我们 研究了一种新的合成肽和一种PLA 2抑制剂的作用， 抗炎蛋白，对大鼠EIU。

Identity and topographic localization of immunocompetent cells and alteration of surface markers on ocular resident cells in rodents with experimental autoimmune uveoretinitis (EAU) were analyzed by immunohistochemical studies. In the early stage of EAU, T helper/inducers were the predominant cells in the eye; in the late stage, a relative increase of T suppressor/cytotoxic cells was observed. T lymphocyte specificity is directed to small fragments of antigen bound to cell surface major histocompatibility complex (MHC) molecules which are presented on the surface of specialized antigen-presenting cells. Expression of MHC class II antigens was observed on ocular resident cells such as RPE, retinal endothelium, keratocytes, fibroblasts, and ciliary epithelium. Both the infiltrating cell subpopulation and the expression of class II antigens on ocular resident cells can be altered by various immunomodulating agents, some of which-Qingaining, FK506, 15-deoxyspergualine, interleukin 2-PE40, and S-antigen-were evaluated for their immunopathological effects on EAU. Clinical and immunopathologic examinations of the eyes of mice with EAU demonstrated a chronic relapsing focal granulomatous inflammation and vasculitis in the retina and choroid. Development of subretinal neovascularization may also occur. The infiltrating cells were mainly macrophages and T helpers/inducers (CD4). The expression of MHC class II antigens is confined to ocular resident cells immediately at the inflammatory sites. Due to the chronicity and recurrence of EAU, the mouse may serve as a better model for human ocular inflammation. Experimental endotoxin-induced uveitis (EIU) is a model for anterior uveitis in humans. The mechanism of this inflammation is still unclear. Because recently it has been suggested that activation of phospholipase A2 may play a role in the initiation and propagation of this disease, we studied the effect of a novel synthetic peptide and a PLA2 inhibitor, antiflammin, on EIU in rats.