PHYSICAL MECHANISMS OF PULSED LASER MICROBEAM INTERACTIONS WITH CELLS

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 使用时间分辨明场，相差和荧光显微镜的方法，我们正在调查的物理机制负责细胞裂解，细胞手术，以及分子传递到单个细胞（光注射）或整个细胞群体（optoporation）。这些时间分辨技术允许在纳秒级的时间尺度上可视化等离子体形成、冲击波传播、空化气泡形成和破裂等过程。此外，使用外源性荧光报告使我们能够确定时间分辨的细胞反应的微束扰动。目前，研究集中在PtK 2细胞系上，使用532 nm激光波长，脉冲持续时间在200 ps-5 ns范围内。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Using time-resolved brightfield, phase contrast and fluorescence microscopy methods we are investigating the physical mechanisms responsible for cell lysis, cellular surgery, as well as molcular delivery to individual cells (optoinjection) or entire cell populations (optoporation). These time-resolved technqiues allow for visualization, on time scales as rapid as nanoseconds, processes such as plasma formation, shockwave propagation, cavitation bubble formation and collapse. Moreover, the use of exogenous fluorescence reporters enable us to determine the time-resolved cellular response to the microbeam perturbations. Currently, studies are focused on the PtK2 cell line using the 532 nm laser wavelength with pulse durations in the range of 200ps - 5ns.