EVALUATION OF ANALYTICAL COUNTERCURRENT CHROMATOGRAPHS

两种逆流色谱模型的性能，高速 逆流色谱仪（HSCCC-4000）和分析环形线圈 离心分离机（TCC），根据理论塔板数进行评价， 分离度和分离时间，以评估其分析 能力。进行了一系列实验以研究 螺旋柱的内径和长度以及流速的影响 移动的相在两相溶剂中分离吲哚生长素的研究 正己烷/乙酸乙酯/甲醇/水组成的体系， 体积比吲哚生长素的三种组分完全相同， 在16分钟内解决了配备有多层线圈的HSCCC系统， 0.55 mm ID PTFE管，理论塔板数范围为829 - 1，290。 用配备有以下设备的TCC系统在24分钟内实现类似的分离： 0.3mm ID PTFE管，理论塔板数范围为969 - 1，811。它 得出的结论是，这两个系统具有可比的分析能力， 发展的现阶段。

Performance of two countercurrent chromatographic models, high-speed countercurrent chromatograph (HSCCC-4000) and analytical toroidal coil centrifuge (TCC), is evaluated in terms of theoretical plate number, resolution factor and separation time to assess their analytical capability. A series of experiments was conducted to investigate the effects of internal diameter and length of the coiled column and flow rate of the mobile phase on the separation of indole auxins in two-phase solvent systems composed of n-hexane/ethyl acetate/methanol/water at different volume ratios. The three components of the indole auxins were completely resolved in 16 min with the HSCCC system equipped with a multilayer coil of a 0.55 mm ID PTFE tube with theoretical plates ranging from 829 to 1,290. Similar separation was achieved in 24 min with the TCC system equipped with a 0.3mm ID PTFE tube with theoretical plates ranging from 969 to 1,811. It is concluded that both systems have comparable analytical capability at the present stage of development.